Transformation And Function Analysis Of A Steroid 5 Alpha-Reductase Gene (GhDET2) In Cotton (Gossypium Hirsutum L.)

Cotton (Gossypium hirsutum L.) is the most important worldwide fiber crop and is vital for textile industry. Although the traits of cotton have been improved in the past several decades by traditional breeding methods, the gene resource that can be used in cotton was greatly restricted by the sexual incompatibility in different species. On the other hand, gene engineering provides a brand-new approach by introducing useful genes from various organisms into cotton.Brassinosteroids (BRs) are a special class of plant hormones. They play an important role in the growth and development of plants, involved in the process of cell division, elongation and redifferentiation, pollen tube germination. Also they can help plants relieve or overcome environmental stress.The steroid 5 alpha-reductase encoded by DET2 is important in the biosynthesis of brassinosteroid. The phenotype of det2 muatnt showed dwarfism, de-etiolation and loss of light regulation function. This paper is concerned with the transformation of GhDET2 cloned from cotton fiber. By overexpressing the GhDET2 gene, we expect to increase the content of endogenous brassinosteroid of transgenetic plants to improve the quality of cotton fiber. The main results are as follows: 1. The investigation of factors affecting transformation for embryogenic callus of cottonAmong three Agrobacterium tumefaciens strains employed, the frequency of GUS expression in the callus infected by EHA101 and EHA105 was higher than that of LBA4404. However, EHA105 and EHA101 grew too fast to control. For the methods cotrolling...