| BMP gene affecting growth and development of cow has been researched. Using the sequence information of other species in the Genbank, through megalign, analysed it`s sequence and method of PCR-RACE,we magnified cloning and the sequence analysis of the cow BMP-4 that didn′t report yet. The method was: first, searching for the BMP-4 gene sequence of human, rat and sheep in the Genbank, we analysed the homology of the BMP-4 gene sequence by using DNAStar software. According to the homology of the longer exon sequence we designed the primer. We used the classic method to extract the genomic DNA from the cow blood, adopted PCR technique to magnify the parts of sequence of the cow BMP-4 with it as template, and reorganized this fragment to the PMD18-T vector, slided it by two different enzymes and PCR identification, we acquired a part of exon sequences of the cow BMP-4 for 306 bps. Second, we designed the different primer of the RACE upper stream through BMP-4 exon sequence, extracted the total RNA of the cow cartilage using the TRIzol Reagent, then PCR-RACE according to the Oligo(dT)15 Adaptor primer and upper different primer. We used the technique of clone and analysed knowing the sequence including 3′ un-translated sequence and mostly coding sequence, the length was 1306bp. The aa(amino acid) of 3′ including of seven cysteines that is the character of BMP gene. On the basis of comparing the sequence to human, sheep, rat, mouse, dog and chicken, the finding homology were 90.2%, 94.2%, 90.9%, 90.4%, 85.4% and 73.6% respectively. We got a result that this cloned sequence is the BMP-4 sequence of cow. At the same time, we analysed the amino acid sequences. The result was the cloned fragment codes 389 amino acids, the length of 3′ un-translated region is 121 bps. The homology in human, sheep, rat, dog and chicken is relatively high. It is important basis for cloning other BMPs family. This sequence has been registed in the Genbank, and the number is AY854957.
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