| Rabies is a kind of zoonotic viral disease whose appearance has been notable throughout recorded history. It is the most popular and most severest infection disease in the world. WHO reported that there are 35,000-70,000 deaths each year worldwide. And the losses of domestic animal and other animal deaths are unable to estimate. In recent years along with molecular biology and immunology development and application, we know all aspects of rabies virus , such as virus structure, the gene sequence and its the main function and so on. In this study, the cDNA fragments of L gene 5' terminal of two rabies virus strains in Jilin and Henan were cloned and sequenced with method of RT-PCR and 3'-RACE . The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5' terminal( NCS),and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I. And comparison of the nucleotide sequence of L gene 5' terminal for deep research. It shows that the lowest homology among these rabies virus strains is DRV8202 and MRV, the homology is 64.8%. Statistics of each kind of basic group of NCS indicate that the contents of G+C% are all taller than 38.89%, contents of G+C% in DRV8202 is 51.85, and 42.95% in MRV. But each kind of basic group composition content is difference. Most of amino acid are basic amino acid in LP, two domains in LP are hydrophobic region (be situated at 851~869 and 1962-1980) ,they maybe participate in interaction of LP and N, M1。 Through comparison, Met is mutated into Val at the site of 856 in the DRV8202 strain, Ala is mutated into Ser at the site of 1971 in the MRV strain, there are not mutations in other amino acids. |
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