Cloning And Sequence Analysis Of P1 Gene Of Inner Mongolia Vaccine Strain Of Foot-and-Mouth Disease Virus Type O

The total RNA of virus was extracted from the Inner Mongolia vaccine strain of FMDV type O, and the RNA extracted was used for template to amplify P1 gene by Reverse transcription- polymerase chain reaction. Then, the amplified DNA fragments were cloned into plasmid vector pGEM-T Easy and transformed E.coli DH5α competent cell. A positive recombinant plasmid which contained whole P1 gene had been confirmed by PCR and sequencing. After that, the sequences were connected together by SepMan software of DNAStar Software package. The Sequence Distances of P1 gene between the gained sequence and other published sequence of FMDV corresponded and analyzed by the software of DNA Star, and Drew the Phylogenetic tree. Analysis result showed that the Sequence Distances of P1 gene between the Inner Mongolia vaccine strain of FMDV type O and O/UKG/35/2001,,O/SAR/19/2000, O/Tibet/CHA/99 is 98.6%,98.7%,99.1%; but, the Sequence Distances of P1 gene between the Inner Mongolia vaccine strain of FMDV type O and O1K,O1/Campos,O/AKS/58,O/Yunlin/TaiWan/99 is only 83%～86.5%.The Inner Mongolia vaccine strain of FMDV type O belongs to PanAsia Genetic group. The main diferrance between the Inner Mongolia vaccine strain of FMDV type O and other FMDV at 547～573,656～681,720～734 Amino acids of P1 gene, particularly at 656～681 Amino acids is namely 130～160 Amino acids of VP1. This study provide the dependable data for the further research on Molecular structure, the relation of heredity evolution and genetic engineering vaccine of foot-and-mouth disease virus.