Cloning And Sequence Analysis On E2 Gene Of Classical Swine Fever Virus Of The Prevalent Virulent Strain In Inner Mongolia And "C"Vaccine Strain

In this study, The E2 gene nucleotide sequences of Prevalent Virulent Strain of classical Swine Fever (CSFV) collected from Inner Mongolia were amplified by reverse transcription polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (nPCR), then, the amplified fragments were cloned in PMD-18-T vector and sequenced. The nucleotide sequences and the deduced amino acid sequences of the Inner Mongolia strain (NMFL) and C-strain were compared and analyzed by DNAstar software. The further study of genetic relationship between the major protective antigen encoding regions of E2 genes of Prevalent Virulent Strain and CSFV C-strain was done by constructing the phylogenetic tree . The results showed that : 1 The E2 gene of NMFL strain and C-strain were all 1170bp in length , they contain total signal peptide sequence and part transmembrane domain structure sequence. 2 The homology of NMFL strain and C-strain were lower which were 82.6% for nucleotide sequence and 87.9% for deduced amino acid sequence. 3 The NMFL strain and C-strain were classified into different subgroups in the phylogenetic tree because of their lower homology , meanwhile, the homology of NMFL strain and other reference strains were high ,so they were classified into group 2 in the phylogenetic tree, and the C and SHIMEN strain were classified into group 1 in the phylogenetic tree.