Classical Swine Fever Living Cell Vaccine In The Production Detect BVDV,PPV,PCV2 And Mycoplasma PCR

The vaccine of Classical swine fever plays a very important role that prevention,controlling and eliminating the disease.The most commonly used vaccine for Classical swine fever is the bovine testicular living cell vaccine.In the production,the vaccine enterprises should prevent the contamination of the raw material,cell,plant,virus,and finished products,so as to avoid the effect and safety of the live vaccine.The rapid and accurate test of the exogenous microorganism is to improve the production efficiency of the live vaccine,reduce the production cost,and ensure the safety and effectiveness of the vaccine.The classical method of culture is the standard method for the screening of microbial contamination of exogenous microorganisms.But the method has some limitations.PCR detection is an emerging molecular biological detection technology,which has the advantages of fast and accurate,the application of PCR detection in the production of classical swine fever vaccineIn this study,A comparative test was conducted for the detection of BVDV,PPV,PCV2 and mycoplasma of the exogenous virus of Classical swine fever vaccine.The results showed that the PCR method was consistent with the classical method results.There were 68 samples tested by exogenous virus,and one batch of positive and cell culture was not consistent with the PCR test,and the other were consistent with the other.The PCR test of 47 mycoplasma samples showed that the positive and the culture of the new serum were not consistent with the test.The reason may be that the sensitivity of PCR and the death of mycoplasma can also detect the false positive.The specific test results showed that the other samples were negative,indicating that the PCR method was good in specificity.The test cycle of PCR is only 2-4 hours,while the training period is 17-29 days,and it can be operated several times during the period.The PCR test is sensitive,specificity and easy to determine.Not only the result of the cultivation method requires some experience,but also influenced by subjective factors,resulting in misjudgment.In 2016,The PCR detection method was applied to test the BVDV,PPV,PCV2,and mycoplasma in 234 batches of cattle testis,46 batches of serum and 120 batches of finished products.The results were in accordance with the standard training method.In the process of production,the PCR method was used to track and detect the BVDV.and the positive rate of cattle testis cells was 8.7%.The positive rate of cattle testis cells was 8.4%,and the positive rate of Classical swine fever was 22.6%.because the BVDV Bovine-derived virus,Due to sampling and assay low levels of virus,detection not to come out,but pass from generation to generation,virus reproduction,appear the same turn some bottles of BVDV,while others have.The quality of the finished products is guaranteed by the timely treatment of the positive items to ensure that the unqualified raw materials and semi-finished products are not flowing into the next process.At the same time of classic culture method of detection,and on-line detection using polymerase chain reaction(PCR),improve the ability of exogenous microbial detection,reduce costs,improve efficiency,ensure the safety and efficacy of Classical swine fever vaccine.