Clone Of MOC1 Promoter And Its Inductive Material Selection In Rice

Tillering in rice is one of important agronomic trait that determine grain yield. Tillering is also of developmental importance because tiller is special type of branches which is quite different from that of dicot plants. M0C1 gene can control formation of axillary meristematic tissues and tillering bud in rice, and its subsequent outgrowth. So, M0C1 is a gene that is important in the control of rice tellering.We aimed in this study to construct plant transient expression vector with M0C1 promoter and convenient vector. Then, induce the GUS gene expression by chemical material in latex. So that we can choose the chemical material that M0C1 promoter can respond.Two 5' upstream promoter region of M0C1 gene were isolated by the method of PCR from genomic DNA of rice, one fragment is 761bp(MOCP4), another is 1961bp(M0CP6). DNA sequence analysis and homology comparison indicated that the two promoter fragment have 100% and 99% homology with reported sequence that has been submitted to GeneBank. We analysised the core promoter region with software of Promoter Proscan Version 1.7, the result indicated that the two fragment have core promoter region in 639bp to 389bp before initial code ATG of M0C1 gene. After this, we analysised the upstream regulatory elements in the two promoter fragment with software of Plant Care, and found lots of light- responsive element, and abscisic acid-responsive element, auxin- responsive element, ethylene-responsive element, gibberellin-responsive element, salicylic acid-responsive element.The CaMV 35S promoter of pCAMBIA1301 was replaced by two 5' upstream promoter fragment of M0C1 gene, and constructed two plant transient expression vector including GUS gene: pCAMBIA1301-M0CP4 ( 761bp) and pCAMBIA1301-MOCP6(1961bp). We put plasmid of pCAMBIA1301-M0CP4, pCAMBIA1301-MOCP6 and pCAMBIA1301 vector into latex to induce expression of GUS gene with Chlormequat Chloride(CCC), Paclobutrazol(PP333), ABA, GA3, IAA, NAA, KT, 2, 4-D and ethanol. The result showed that two promoter fragment can be induced by GA3, NAA, KT and CCC. The inductive activity of two promoter fragment no discernible difference for NAA, KT, CCC. But the...