I-Chintinase Gene Driven By Pathogens Responsive Promoter Genetic Transformation Of Sugarcane

Sugarcane(Saccharum officinarum L),which is the most important industrial material crops in China, is affected by pathogens because of abscence the disease resistant sugarcane cultivals.The most important diseases are caused by fungi,eg Ustilago Scitaminea Syd.However sugarcane is a nonsexual propagation crop with highly ployploidy and complex genetic background,breeding pathogens-resistance cultivals by using traditoonal breeding techniques will be a formidable task.In contrast,genetic engineering enables modification involving the eotipic expression of genes from non-plant sources,which wii be an alternative pathway.A plant expression vector,pBPC,was constructed by ligation a pathogeny responsive promter,prp 1-1,from patato,and I -Chintinase gene from tabacco.It was introduced into the super-poison Agrobacterium tumerfaciens EHA105 through "Triparental mating"method.Sugarcane, embryonic calli was transformed by Agrobacterium tumerfaciens-mediated method.The transformed shoots were screened under the selective pressure of G418 and 531 resistant were obtained. The method of PCR was used to detect the I -Chintinase gene and 5 PCR-positive plants in the 30 resistant shoots.The integration of foreign DNA into the genome of a transgenic plant was confirmed by Southern blotting analysis.