Studies On Preventing And Curing Silkworms Muscardine With Terbinafine·HCL

Terbinafine.HCL is one of acrylamine antifungal. In vitro activity antifungal agents of Terbinafine.HCL was determined with National Committee for Clinical Laboratory Standards (Reference method for broth dilution antifungal susceptibility testing of conidiumforming filamentous fungi.Proposed Standard M38-P). Biologic test of Pharmacodynamics and physiological influence was done. The pharmacokinetic study of Terbinafine.HCL were conducted by using the High performance liquid chromatography (HPLC). Pharmacokinetics parameters were analyzed with the analyzing pharmacokinetic program of 3P87. The experiment to prevent and cure silkworms muscardine and silkworms bacterial disease were conducted with Terbinafine.HCL and Erythromycin together. The inhibited experiment in vitro showed that the three pathogenies of silkworm muscardine were sensitive to Terbinafine.HCL. The MIC range of TBF was 0.0625～0.2726μg/mL ,the MFC range of TBF was 0.125～4μg/mL. The MIC range of FCZ was 0.25～64μg/mL ,and FCZ hadn't disinfection. Biologic test showed that Terbinafine.HCL was good at controlling silkworm muscardine. Terbinafine.HCL's concentration of 200 μg/mL had a complete control. Terbinafine.HCL showed fine stability. Pharmacokinetics of Terbinafine.HCL to silkworm consulted the method of higher animal. The concentration of Terbinafine.HCL in silkworm plasma were determined by HPLC. Methanol was done to subside protein of silkworm plasma. Protein was almost subsided after high speed centrifugation, secondary sedimentation, filtration with microporous membrane. The mean recovery rate in the test was 94.3%±2.47%. It showed that this method was reliable. Pharmacokinetics parameters were analyzed with the analyzing pharmacokinetic program of 3P87. The results showed that the concentration-time course of Terbinafine.HCL in plasma following adding adminidtration in silkworms (25 mg/kg) could be described by one-compartment open model with first order absorption. The primary parameters wee showed below: :Ka=0.904 h^-1, Ke=0.545 h^-1, Cmax 13.467 h, Tmax=1.803 h, T.Ka=0.766 h, T.Ke=1.272 h. These parameters showed that Terbinafine.HCL has many characteristics, for example, it could be absorbed...