Research On Self-(in)compatibility And Other Yield Components In The Apricot F₁ Progenies

In order to provide scientific basis for apricot (Prunus armeniaca Lam.) breeding and related research, inheritance and correlation of yield components including self-compatibility, self-pollinated fruiting rate, fertile flower rate, average fruit weight and fruit number per plant were studied with 4⁵ years old seedlings of apricot F₁ population from Katy ×Xinshiji, Katy ×Hongfeng and Katy ×Taianshuixing. And the sequences of the S allele gene in Katy, Xinshiji and some F₁ progenies were obtained and analysised. The results were as follows: 1. According to the standard that self-pollinated fruiting rate in self-compatible cultivars was higher than 6%, the ratios of self-compatible to self-incompatible individuals were 27:25, 9:12 and 15:19 in the above three families, respectively, and they were proved to be a 1:1 segregation by X² test, so it could be concluded that the self-compatibility of Katy was transferable and heterozygous, and self-compatibility was dominant to self-incompatibility. 2. Thirty-eight seedlings from the F1 population of Katy(ScS₈)×Xinshiji (S₉S₁₀) were amplified by S-allele-specific PCR, obtaining four S-genotypes with the ratio of 21 (ScS₈,S₈S₉): 9(ScS₁₀): 8(S₈S₁₀) which were confirmed to 2:1:1 expected ratio by X² test. Therefore, we could conclude that the inheritance of S allele gene is agree with the Mendel theory. 3. The S allele genes of Katy, Xinshiji and 9 their F1 progenies selected were cloned and sequenced. Three new apricot S allele genes were obtained and had succeeded enrolled in GenBank. And great differences in the degree of self-compatibility were observed among the seedlings with the same S-genotype, indicating that self-compatibility in apricot was quite complicated and dominated either by S-gene, S-genotype or other genetic factors. 4. In the F1 populations, a very extensive segregation in self-pollinated fruiting rate, fertile flower rate and average fruit weight was observed, and...