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Study On Genetics Of Biology Parthenogenetic Induction DH Lines And Resitance To Stalk Rot In Maize

Posted on:2006-01-08Degree:MasterType:Thesis
Country:ChinaCandidate:Y Q LiuFull Text:PDF
GTID:2133360152991984Subject:Crop Genetics and Breeding
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The double haploid (DH) lines derived from maize parthenogenesis 1145×Y331 F1 were used to study genetic segregation with SSR markers and the agronomy traits. Since 1145 is stalk rot resistant line, and Y331 is stalk rot susceptible line, the DH lines were also planted in two replicates to inoculate the stalk rot pathogen F. graminearum and P. inflatum separately to study the genetic model of resistance . The results were as follows:1. Significant genetic variation were found among DH lines, plant height, ear height and kernels per row showed normal distribution, and ear length showed multi-peak distribution. The CV% of morphological traits in DH lines were significantly smaller than that of R1L, which confirmed that the DH lines were identical.2. Ninety-two DH lines were assayed with 32 SSR markers. The segregation of 1145 and Y331 did not deviate from the ratio of 1:1. Comparing genetic segregation of SSR markers in haploid and double haploid population, no significant difference were found, which showed that there were no gametic selection in haploid inducing and doubling.3. The resistance to stalk rot pathogen F. graminearum and P. inflatum in DH lines indicated same tendency, which meant that the resistance to the two pathogen could be controlled by same gene the resistant ratio did not deviate from the ratio of 1:1. Results showed that tassel date, ear height and ear length had significant relation with stalk rot resistance, the line with short ear , higher ear height and late tassel date had stronger of resistant ability to stalk rot.
Keywords/Search Tags:maize, SSR, DH lines, inheritance, stalk rot
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