Analysis Of Expression Profile Of Cryphonectria Parasitica By Using ESTs And CDNA Microarray

Hypovirus/ Cryphonectria parasitica has proven to be a tractable system for studying the interaction between virus and host. In order to gain a better understanding of the molecular basis for the interaction, a cDNA library was constructed from mRNAs isolated from the wild-type (virus-free) and hypovirus-infected C. parasitica strains at the stage of sporulation. A total of 2403 expressed sequence tags (ESTs) representing 1402 tentative unique transcripts (TUTs) were collected by sequencing the cDNA library. A cDNA microarray representing 4845 TUTs of C. parasitica, of which 3806 TUTs were from non-sporulating stage collected previously and 1402 TUTs from sporulating stage, was spotted on a nylon membrane. The array was used to monitor changes in the expression profile after infection by hypovirus CHV1-EP713. Altered transcript accumulations were observed for 1055 clones, in which 370 genes were up-regulated and 685 down-regulated. The array hybridization results were verified by the real-time quantitative reverse transcription-PCR and it was revealed that 8 out of 10 randomly selected TUTs with altered expression were coincided in trend with the array analysis. The genes whose expression were modulated by the virus cover a broad spectrum of biological processes, including stress response, nucleic acid metabolism, proteinmetabolism, and many others. These findings provide a new insight for the interaction between hypovirus and its host fungus.