| Some principal factors affecting the efficiency on culture in vitro of quality indica rice were studied systematically. At the same time, Agrobacterium tumefaciens could be used to mediate genetic transformation of quality indica rice. The results indicated that:1.The effects on mature embryo sterilization were quite different with different sterilant combinations.70% CH3CH2OH 3 min + 10% NaClO 10 min + 0.1% HgCl2 30 min had optimal effect on mature embryo sterilization.2.Basic media were selected in mature embryo culture. MS media was suitable to callus induction of Guihauzhan. N6 media was suitable to callus induction of YouzhanNo.8.3.2,4—D was a necessary factor in callus induction.2.0—2.5mg/L 2,4—D in callus induction medium were optimal.2,4—D combined with KT/BA/NAA in induction media could express the better efficiency on mature embryo culture.4.Added 0.01—0.05mg/L ABA to callus induction media could increase the callus induction rate and improve the quality of callus.5.500mg/L proline added to callus induction media could increase the callus induction rate effectively.500mg/L proline combined with 500mg/L glutamine was not only increase the callus induction rate, but also decrease the callus browning rate.6.Added 10ml/L CM to callus induction media could increase the callus induction rate effectively. Added 10—20ml/L CM to callus induction media was help to increase embryogenic callus induction and green seedling differentiation.7.The effects of callus induction, subculture and diffe — rentiation were quite complex according to different hormone categories, concentrations and their combinations. The best treatment were 2, 4—D2.0+BA0.5+ NAA0.5 in callus induction media, 2, 4—D2.0+KT1.0+NAA0.2 in subculture media and BA2.0+NAA0.5 in differentiationmedia.8.Three antioxidants of 0.1% concentration were used to pretreat for different time on rice mature embryos. Citric acid's effect of inhibiting callus browning was the best, but it would reduce callus induction at the same time. Vitamin C was not only inhibiting callus browning but also improving callus induction and callus growing. PVP had determinate effect on inhibiting callus from browning.9.Used 0.1—0.5% Vitamin C to pretreat rice mature embryos for 3 hours could help to callus induction and growing, improve the quality of callus, and decrease the callus browning rate.10.Added 10mg/L Vitamin C to callus induction media could decrease callus browning effectively.11.Added 60—120mg/L Vitamin C to subculture medium could decrease callus browning and increase the frequency of green seedling differentiation.12.Added a small quantity of mannitol or sorbitol in callus induction media or added proper quantity of mannitol or sorbitol in subculture medium were inhibiting callus browning and improving callus quality.13.The rate of green plantlet differentiation was to the maximum after callus induction for 15 days, subculture for 10—15 days. The callus browning rate was increased with the prolongation time for the callus induction time. The rate of green plantlet differentiation was decreased with the pro- longation time for the subculture.14.There were different requirements with different hormone categories , concentrations and combinations in the test on root induction and sound seeding of embryo plant.15.The subculture times of green plantlet influenced the survival rate of transplanted green plantlet.l6Agrobacterium tumefaciens mediated A208SE genetic tran -sformation the GUS gene of calli ofindica rice, You zhanNo.8, the rate of GUS gene immediate expression was 2.44% -12.00%, the differentiation of green plantlet was 5.00%-15.38%.
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