The Evaluation And Application Of Rice Storability Genes

Lipase and lipoxygenase play important roles in seed aging. The effects of theabsence of lipase and lipoxygenase on rice storability were achieved throughoptimization of artificially aging conditions and evaluation on rice storabilitycharacters. 1,Further studies were carried out on rice artificially aging conditions.Comparative studies were conducted between three rice artificially aging methods:38℃,RH60～65%,42days;38℃,RH85%,42days and 42℃,RH88%,24days .Their effects on rice storability were compared. The results showed that the optimizedartificially aging conditions were 42℃,RH88%,24days This method is quick andaccurate in evaluating rice storability. 2,Different genetic rice has significant vitality differences after 12day storage at42℃,RH88%.12days can be a key time at 42℃,RH88% for choosing elite storablerice varieties. 3,For different genetic rice seeds, they begin deteriorating severely at differenttime. At 42℃,RH88%,for different rice seeds the corresponding time may be LOX-3null or common 8 days, LOX-1,LOX-2 null or lipase null 12 days; LOX-1,LOX-2,lipase null 20 days. 4,Scanning electronic mirror photos show that the absence of lipase or LOX-1,LOX-2 can starve the rice from deterioration effectively and had a less severe damageto the cell membrane system. 5,With seed aging at artificially aging conditions, the seeds with the absence ofLOX-1,LOX-2 and lipase have the best storability while common seeds or those withLOX-3 null have the poorest storability and those with the absence of LOX-1,LOX-2or lipase have better storability. 6,Among three lipoxygenase Isoenzymes , the lower content LOX-1,LOX-2may play key roles in seed superoxidation. The absence of LOX-1,LOX-2 canprevent seed lipid from superoxidating more effectively than that of LOX-3.