Identification Of The Soybean Allergenic Protein And Studying Of Its Genetic Regularity

Soybean protein is an important protein in the food industry because of its excellent nutrional. Butsoybean protein contain multiple allergenic proteins that can make an allergic reaction. At present, thegrowing consumption of soybean products in all over the world may result in an increase in the incidenceof patients with soybean allergy. In order to identify the soybean varieties that lack of allergenic soybeanprotein, and to reveal genetic distribution and function, 481 soybean accessions that from soybean corecollection, reserved collection and wild soybean respectively were selected and identifed by immunologymethod. Also, SSR markers was used to analyze the relationships between accessions with and lack ofallergic protein.The major conclusions are as follows:1. The ratio of lacking of Gly m Bd 28K in soybean accessionsis lower in North than in south of China.The average ratio was 37.7% in cultivar. there was no significant difference between Huanghuai summersoybean and South soybean at the lacking ratio, while they both significantly differed with North springsoybean. The lacking ratio of Gly m Bd 28K in Landraces (40.4%)was slightly higher than that of inbreed varieties.2. The content of allergenic proteins (Gly m Bd 28K and Gly m Bd 30K ) in soybean was aboundant, butit was not correlative with that of rough protein.3. The ratio of lacking of Gly m Bd 28K in core collection, reserved collection and wild soybean were36.3%, 39.3%, 13.3% respectively. there was was no significant differences between the core collection andthe reserved collection, while they both significantly differed from wild soybean.The distribution law ofabsence Gly m Bd 28K of core collection and reserved collection in each ecotope was almost the same,showing that core collection was representative.4. Germplasm of absent Gly m Bd 28K had abundant genetic variability (Simpson value:0.801)and lowgenetic similarity. The core collection had more wide genetic basis and the mean similarity coefficient(0.186)was lower than that in the reserved collection . We can arrange in pairs in or between genus inabsent Gly m Bd 28K germ plasms.5. The cluster results showed that accessions in same ecological region group together by molecular dataand accessions in same producing area clustered in the same sub-group and the seed color of accessionsthat in the same sub-groups were almost similar. In addition, when accessions in the core collection and inthe reserved collection were clustered, there was no significant limit between them on the dendrogramt.6. Traits that lacking of Gly m Bd 28k was controlled by single recessive gene.And the gene's inheritancelaw agreed with mendelian inheritance law. The results of reciprocal cross were coincident, which indicatedthat Gly m Bd 28k was controlled by nuclear gene. Accessions would lack of Gly m Bd 28k, when a purerecessive allele was present.