Purification And Structural Identification Of Herbicidal Active Fraction From Metabolites Of Botrytis Cinerea

It is well known that the compatible green pesticide with environment has been the inevitable direction of the development of pesticide formulation in the contemporary era, it is one of the important approach on research green pesticide that screen active pesticide substances or chemoinformatics in millions of crude compounds. The fungi Botrytis cinerea is a known pathogen of tomato gray mould, an isolate of B. cinerea metabolites was used to research as green pesticide in this paper.(1)BC-4 isolate of B. cinerea had been mutated by 30W ultraviolet lamp illuminating for 60min away from 30cm, and isolate 7-3 was selected from 36 mutative single-spore strains. The concentration of weeding activity metabolites in it's cultured filtrate was the highest. The better condition to produce high active metabolites from culture filtrate of isolate 7-3 was constructed based on the results of bioassay and HPLC analysis. That include inoculating isolate 7-3 into modified PD liquid media of initial pH 4.0, and culturing 18 days under dark and static in the procedure of rise temperature from 18℃ to 28 ℃.(2)An active herbicide ingredient was obtained from culture filtrate of isolate 7-3 by freeze drying, extracting and dissolution in different solvent and so on. Bioassay results indicated that the ingredient has selective activity on different weeds but safety to cereal crops. The inhibitions to weeds Echinochloa crusgalli, Setaria viridis, Chloris virgata, Eleusine indica, Descurainia sophia, Capsella bursapastoris, Arabidopsis thaliana were higher than Amaranthus retroflexus, Portulaca oleracea, Chenopodium album. The death percentage of the fomer is above 80% but the later is approximate 40%. The ingredient can also produce big acreage necrosis spot in leaves of tomato, cucumber and jowar, but no effect on wheat, maize and cotton. Thus, the ingredient may contain some herbicidal components.(3)Five different ultraviolet absorption components were isolated from the ingredient of cultured filtrate of isolate 7-3 by TLC, CC and HPLC. Of the five components, one...