Establishing Of A Rapid Shoots Multiplication Procedure For Early Ripe Nectarine (Prunus Persica Var Nectaria) And The Study Of Factors Affecting Shoots Organogenesis From Hypocotyls

From　the　study　of　plant　growth　regulators　and　their　combination,　base　media,　darkening　treatment　and　other　factors,　we　have　developed　a　rapid　shoot　multiplication　procedure　for　early　ripen　nectarine　(Prunus　persica　var　nectaria).　The　effects　of　plant　growth　regulators,　base　media,　darkening　treatment　and　the　developmental　stages　of　embryo　were　investigated.　We　have　developed　a　shoot　organogenesis　from　hypocotyls　procedure　for　early　ripen　nectarine　(Prunus　persica　var　nectaria).　Shoots　of　Prunus　persica　var　nectaria　cv.　Huaguang　were　placed　onto　shoot　induction　media　such　as　G+TDZ　2.0mg/L+KT　1.5mg/L　and　G+6-BA　1.0　mg/L+KT1.0　mg/L,　incubating　directly　under　cool-while　fluorescent　lights.　A　maximum　of　100%　shoot　showed　multiplication.　And　the　means　number　of　effective　shoots　per　explants　are　17.5　and　16.31.Shoots　of　Huaguang　were　placed　onto　root　induction　medium　1/2MS+IBA2.0mg/L,　incubating　directly　under　cool-while　fluorescent　lights.　Multiple　inductions　of　roots　inducted　from　shoot　base　within　20　days　culture.　The　rate　of　rooting　is　100%.　The　means　number　of　effective　roots　per　explants　is　7.17.　Shoots　of　Prunus　persica　var　nectaria　cv.　Shuguang　were　placed　onto　shoot　induction　medium　G+TDZ　2.0mg/L+KT1.5mg/L,　incubating　directly　under　cool-while　fluorescent　lights.　A　maximum　of　100%　shoot　showed　multiplication.　And　the　means　number　of　effective　shoots　per　explants　are　9.93.　Shoots　of　Huaguang　were　placed　onto　root　induction　medium　1/2MS+IBA2.0mg/L,　after　9　days　of　darkening　treatment,　incubating　under　cool-while　fluorescent　lights.　Multiple　inductions　of　roots　inducted　from　shoot　base　within　20　days　culture.　The　rate　of　rooting　is　100%.　The　means　number　of　effective　roots　per　explants　is　7.17.　If　shoots　were　darkening　incubated　longer　than　9　days,　they　often　etiolated,　so　the　plants　grow　abnormal.　The　lower　concernment　of　IBA　(<2.0mg/L)　can　induct　fibrous　roots.　If　the　concernment　of　IBA　is　higher　(>2.4mg/L),　many　callus　were　inducted　in　the　base　of　shoots.　And　the　roots　were　inducted　from　the　callus.　74DAFB　is　the　better　stage　of　embryo　culture.　Embryos　of　60DAFB　and　81DAFB　could　not　germinate.　Removed　seed　coats　and　75　days　cool(5℃)　treatment　are　benefit　to　embryo's　germination.　Hypocotyls'　upper　part　of　morphology　has　the　higher　ability　of　regeneration.　Hypocotyls　of　Huaguang　were　placed　onto　shoots　induction　medium　G+TDZ　3.0　mg/L+　KT　1.0　mg/L+NAA　3.0　mg/L,　incubating　directly　under　cool-while　fluorescent　lights.　A　maximum　of　23.43%　shoots　showed　multiplication.　And　the　means　number　of　effective　shoots　per　explants　is　1.08.　If　they　were　cultured　onto　QL+TDZ0.5mg/L+KT2.0mg/L,　the　maximum　of　2.01　means　number　of　shoots　organogenesis　per　explants　was　obtained.　Hypocotyls　of　Shuguang　were　placed　onto　shoots　induction　medium　G+TDZ　2.0mg/L+NAA0.5mg/L,　incubating　directly　under　cool-while　fluorescent　lights.　A　maximum　of　17.47%　shoots　showed　multiplication.　And　the　means　number　of　effective　shoots　per　explants　is　1.08.　If　they　were　cultured　onto　QL+TDZ　3.0mg/L+KT1.0mg/L+NAA3.0mg/L,　the　maximum　of　1.33　means　number　of　shoots　organogenesis　per　explants　was　obtained.