The Study On Calcium Overloads And Chicken E.tenella Injury Mechanism

The purpose of this paper is to study the relationship between Ca2+ and injury of host cell .The experiment examined the change of calcium, sedimental calcium, Na+-K+-ATPase. Ca2+-Mg2+-ATPase , the total ATPase, pHospHolipase A2(PLA2). Free Fatty Acid in caecum tissue and dissociative Ca2+ in caecum epithelium after infection.The result include four parts:(1) The caecum total calcium level of infection group declined remarkablely (P<0.01) and could go down to 22.17±0.29(nmol/gprot) after 5×10~4 E.tenella infecting.Von Kossa calcium coloration indicate the content of calcium in caecum epithelium have a obvious increase(P<0.01). In the process of the E.tenella disease, the content of dissociative Ca2+ have a obvious increase(P<0.01).(2) The activity of Na+-K+-ATPase, Ca2+-Mg2+-ATPase. the total ATPase in caecum tissue all decreased at different degree with the developing of the disease. In the serious pHase,the content is lower than the uninoculation control group(P<0.01) and could go down to 1.60±0.34(U/mgprot) , 3.18±0.94(U/mgprot), 3.91±0.67(U/mgprot) respectively,at the same time the Ca2+ quantity in the cell became increased .The cause of Ca2+ overload should be the injury of Na pump. Ca pump, total - ATPase.(3) The overloads of calcium lead to PLA2 and the content of free fatty acid all went up obveriously and could got to 22.21±2.93(U) and 272.00±3.56(umol/gprot) respectively.It is concluded that calcium overloads acts as an important injury mechanism in chicken E.tenella disease.(4) In the early stage of E.tenella infection, nitric benzene could interdict the entering of the parasite, it could ease the injury of the host cell and have certain protection to the chicken body ,but with the developing of the disease ,its function descend,but the time of the oocyst discharging was prolonged.