The Isolation And Identification Of Avian Reovirus

Avian reovirus infections spreading in chickens cause serious economic problems for commercial poultry producers throughout the world.The acute form of the disease is apparent in chickens between 5 and 7 weeks of ages,in which morbidity is high and the rate of mortality is 1% or more. Recently,with the increase of avian reovirus infection and influence , with the vaian reovirus infection and its influence and with the variety of clinical reaction pattern.It is more and more difficult to prevent avian reovirus infection.To prevent avian reovirus infenction on the point,it is necessary to analyse and study the stereotypeof avian reovirus.In recent years, scientists have done much research about the antigenity and serotype of the pathogenic Avian reovirus in China.In this research,a Fujian avian reovirus isolate(ARV-CL) was studied in the experiments of etioloty,virus-neutralization and genetic analysis of S3 gene.Now it is important to review the advance of the study of its etiology, pathogeny,physicochemical properties,and serial and antigenic relation among ARV-CL, DRV-YB,DRV-YH,and ARV-S1133.This study was useful for understanding the properties of avian reovirus isolates,and also provide important information for the basis of the choice of the vaccine isolates and preventive epidemic plan of avian reovirus in F μ jian province.In this study,four avian reovirus isolates were investigated systematically .We researched ARV-CLisolate's etiology and serology.And its S3 gene were sequenced and analyzed.1 .The sample materials were taken from the fibulo-metatarsal joint fluid andthe edematous tendon of the infected cases that were suspected to bechicken viral arthritis. They were inoculated into the SPF chicken embryosresulting in the isolation of a typical strain CL.2.The viron was shown spherical, no envelope,with double layeredcapsid,about 75nm in diameter by electronmicr oscopy. 3.The virus could multiply on the chick embryo fibroblast(CEF) cell could cause syncytial pathologic change(CPE) with the appearance of cytoplasmic inclusion bodies.The virus strain,passaged by CEF cell, produced evident CPE in 48-72 hours.4.The one-day-old SPF chickens could be infected with the reovirus isolate CL by nasal,oral,intramuscular (i.m.),pad inject- ion.There were inflam matory responses caused by the pad inoculation to the SPF chickens. 5.Heat resistance test and susceptibility of fat solvent and heat resistance test were conducted on virus separately.The lab tests show that under 80°C and 85°C heats the virus died basically in 30 minute and 30 minutes 100°Cheat could destroy the virus completely. PH5 could not affect the virus infection, but PH11 could destroy the infection basically. Ether could not affect the virus infection.6.Four avian reovirus isolates:CL,S1133,YH and YB were checked in the cross-neutralization test. The isolated CL had certain antigen correlation with the other avian reovirus. The result demonstrated that the isolated CL,which was a member of the family Reoviridae,geus Orthoreovirus.7.Phylogenetic analysis of the nucleic acid sequence and the deduced amino acid sequence of CL-S3 demonstrated that CL separated as a distinct virus relative to other avian reovirus,was in far relation to Muscovy duck reovirus strain YB(DRV-YB),and nucleotide and amino acid homogeneity of S3 between isolate CL in China and DRV-YH were 87% and 94.6%,respectively.