The Study Of Induced Protein And Key Enzyme Of Camptotheca Acuminata Seedlings Under Drought Stress

Camptotheca acuminata is a tree unique to China. The molecular ecology of drought stress to Camptotheca acuminata seedlings with the theory and method of functional proteomics was systematically studied. The physiological indexes correlative to drought-tolerance were studied in the laboratory, and the expression of total proteins were also detected in the study.We simulated drought stress with 15% PEG8000, and used 2-DE, PMSF. and N-terminal analysis to identify and analyze the dynamic state of some functional proteins. Spraying Camptothecin(CPT) as a microtubule depolymerization agency, we studied the stoma movement of Camptotheca acuminata seedlings under drought stress conditions.Camptotheca acuminata seedlings could tolerate 15% PEGgooo simulated drought stress during short time but the physiology was severe disturbance. CPT content was ascend under 10% PEGgooo drought stress condition that has important application purport. The results indicated that the molecular mechanism of Camptotheca acuminata seedlings adapted to drought stress on the level of protein included two aspects .Firstly, some drought-induced proteins increased expression such as the Late Embryo Abundant protein (Lea protein) and heat shock protein (HSP), which were significant for maintained mesophyll cell water and normal metabolism. GTPase and Rubisco activase had important funcation on metabolism of Camptotheca acuminata seedlings. Secondly, Rubisco LSU and tubulin were degraded for avoiding self-poisonous denatured protein. It could contribute to enhance mesophyll cell water potential and inhibit the stoma open so as to keep water and adapt to drought stress.