Biological Way On Improving Diosgenin Content In Rhizome Of Dioscorea Zingberensis Wright,Root Yield And Study On Biological Activity Of Dioscin

This paper included three parts. In the first part , based on the field experiment we mainly studied the different effects of various applying ratios and kinds of fertilizer and phytohormone on diosgenin content and yield of roots of Dioscorea zingberensis.Results showed that the degrees of effect on diosgenin content were ranged with magnesium fertilizer (Mg)> manganese fertilizer (Mn) >Urea fertilizer(N)>family fertilizer > amnonium hydrogen-phosphate fertilizer (NH₄PO₄) >phosphate fertilizer(P) >potassium dihydrogen phosphate (KH₂PO₄) >ferrous fertilizer (Fe)>potassium nitrate (KNO₃) >potash fertilizer(K), while the output of dioscorea roots was amnonium hydrogen-phosphate fertilizer ((NH₄)₂HPO₄) > potash fertilizer(K) > urea fertilizer(N)> potassium dihydrogen phosphate (KH₂PO₄) > ferrous fertilizer (Fe)> phosphate fertilizer (P) > family fertilizer > manganese fertilizer(Mn)> potassium nitrate(KN0₃) > magnesium fertilizer(Mg), and the combat ion of was N fertilizer 15 Kg/667m², P fertilizer 50 Kg/667m², K fertilizer Kg/667m², (NH₄)₂HPO₄ 60 Kg/667m², KH₂PO₄ Kg/667m2 superior to others. For phytohormone, the results showed that the degrees of effect on diosgenin content was ranged with 2, 4-D>sweet potao pengdasu > NAA> uniconazol >paclobutrazol, while the output of dioscorea roots was2, 4^D> NAA > paclobutrazol > uniconazol >sweet potao pengdasu.In the second part, different concentration of Ce³⁺was added to study the effects of Ce³⁺ on the tissue culture of excised stem segments and test-tube plantlet propagation and growth of Dioscorea zingberensis were studied. The results of experiments showed that low concentration of Ce³⁺(5-10mg/L) treatments increased callus fomation, root growth rate,the state of growth the number of root and enhance activity of superoxidic dismutase (SOD) , catalase (CAT) and peroxidase (POD) , while high concentration of Ce'i+inhibited them . The bud initiation was inhibited and much more explants turned browing in high concentration of Ce3 + (30mg/L) .In the third part, with regard to the biological activities of dioscin , we studied their activities of scavenging ? OH and. 02"'and. their antimicrobial effects. We also studied their activities on anti-aging and anti-tumour. The results were as follows : Dioscin had remarkably' inhibitory effects on Fusarium oxysporum fsp Vasinfactum, Alternaria alternata and , and it had distinctive effect on clearing 0z ""and ? OH compared with paeonol. The results of anti-aging showed that dioscin could prolong the average age of silkworms , but not obviously (p>0. 05 . Anti-tumour activities showed that dioscin had obvious inhibition to human cervicarcinoma Hela cellsx human breast carcinoma MDA-231 cells^ human sopharyngeal cancer KB cells , but no obvious inhibition to human liver carcinoma 7421.