The Cultivation Of Eperythrozoon Suis In Vitro And The Selection Of Its Effective Drugs

Eperythrozoon suis has not merely brought the enormous economic losses to animal,and it still threats the mankind seriously . The method to cultivate Eperythrozoon suis is unripe in vitro,which has hindered people from studying this disease. And the people know little about this disease at present,which has caused to abuse the drugs to resist Eperythrozoon suis clinically seriously already. The research adopted three methods to study how to cultivate Eperythrozoon suis in vitro: Method one, cultivate Eperythrozoon suis in vitro on the normal red blood cell of eight kinds of animal and the normal red blood cell of person. Method two, cultivate Eperythrozoon suis on the Solid training base or on the liquid training base of Mycoplasma according to the method of the cultivation of Mycoplasma. Method three, cultivate Eperythrozoon suis in the total training base 3, in vitro according to the method of the cultivation of virus on VeroE-6 cell. Observe Eperythrozoon suis under scanning electrical mirror. Train the sediments of thing with PCR to sign whether to contain Eperythrozoon suis. At the same time,select effective drugs in training system of method one in vitro . Eperythrozoon suis can grow and breed on red blood cell of the person and of eight kinds of animal in training system of method one in vitro.The red blood cell of the pig is the most suitable for Eperythrozoon suis to grow on, and its infection rate is the highest, may reach 99%.The red blood cell of Chicken is not suitable for Eperythrozoon suis to grow on, and its infection rate is always lower than 5%. Go down to the future generation on the red cell, may reach more than 36 generations on the red cell of pig(generation/12 hours); may reach more than 18 generations on the red cell of Person, rabbit, little white mouse, goat, sheep and dog (generation/24 hours); may reach more than 24 generations on the red cell of cow. Eperythrozoon suis does not grow on solid and liquid training base or breed in training system of method two in vitro. Under scanning electrical mirror, observe Eperythrozoon suis adhere on VeroE-6 in training system of method three in vitro. But whether to appreciate in large quantities and whether to go down to the future generation do not study. In drugs selecting test, the effect of Fonghongjing is not only rapid, but also good. Comparing with other some kinds of drugs, side effect is also little. Sulphuric acid is invalid for Eperythrozoon suis. Other drugs selected have certain role in Eperythrozoon suis.In a word, the training method of Eperythrozoon suis in vitro makes thorough researchin the disease become possible. The reseach in the drugs selection has offered certain theoretical basis for people to select suitable drugs. At the same time, this research has also offered certain theoretical basis for the classification...