The Research On The F1 Of Population Of Pinus Massoniana With RAPD Markers Technology

Pinus massoniana is belonged to Pinaceae and Pinus L . It is southeast wetness subtropics the region distribute most widely in our country, the resources keeps the most abundant forest of quantity type, also is the special important industry raw material in the southern of our country . Therefore, choosing the Pinus massoniana tree to grow the conduct and actions heredity the resources advantage depth discover with high heredity good kind of the quality wood seed to select for the objects of research, match completely current and future forestry product the development demand and the forestry science and technology request of the economic development.Pinus massoniana loosens to distribute the area inside have numerous rivers with big high mountains, the geographical environment condition variety, because of the long-term nature choice and grow to insulate to wait the reason, exist extremely abundant heredity variation, this material heredity that loosens for Pinus massoniana the improvement provided abundant material foundation.The main purpose of this research is to make use of the loose delicate leaf of Pinus massoniana as material, to exam the first breeding between Pinus massoniana the No. 8 Linduo and the No. Ill of Guangxi are miscell- aneous to hand over the uniform of the prosterity, to adopt new method examing the DNA sequence , carry on the Pinus massoniana loose a RAPD expands table to take the analysis, and carry on the RAPD moleculer markers, authenticating the production application in the characteristic RAPD moleculer markers of a Fl generation;In order to open the loose genetic engineering definite direction of the exhibition Pinus massoniana to grow to lay the dependable foundation, for the purpose of further research and investigate the Pinus massoniana , the loose DNA for express discriminated the technique in early days, shortenning to grow the period, providing a quality of high generation for the production, producing the technique guarantee of provide the credibility for a big area of strong advantage.Making use of the reformative of CTAB method take out to lift the loose leaf...