| Phytoene synthase (PSY), Phytoene desaturase (PDS), Lycopene β-cyclase (LycB), Lycopene ε-cyclase (LycE) are four key enzymes during the course of Carotenoids biosynthesis. The catalysis can be accelerated and the synthesis of relatively carotenoids can be improved through the transformation of related genes. Using leaves and shoot cuttings with petiole as explants, the adaptive acceptor system for genetic transformation was built. Using callus of Anthurium and immature embryo of Phalaenopsis as accepter, PSY,PDS,LycB,LycE genes were transformed into Anthurium and Phalaeanopsis via agrobacterium-madiated and particle bombardment, followed by PCR and PCR-Southern detection. The factors which affected genetic transformation were also investigated. The main results were summarized as followed. Induction of callus and plantlet regeneration were carried out from leaves and shoot cuttings with petiole as explants in Anthurium. The results indicated that the frequencies of callus formation from shoot cuttings with petiole was higher than those from leaves with petiole. The medium containing 1/2MS +6-BA1.0mg/L+ 2,4-D0.1mg/L showed to be the most effective for inducing callus from shoot cuttings with petiole. The frequency of callus formation was improved when KT, 1/2MS and sucrose were applied. Adding 2,4-D or NAA was benefit for inducing root formation. Suitable type of explants was found. The suitable Hyg concentration is 18-26 mg/L. The sensitivity on Hyg concentration varied difference among different vartieties. The genetic transformation system of Anthurium and Phalaenopsis mediated by Agrobacterium tumefaciens was established. The results were as followed: Among three variety of Anthurium, the effect of infection time, concentrations of... |
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