Studies On Exon 8 Mutation Of P53 Gene Of SD Rats' Ovary Caused By ZEN

Zearlenone (ZEN) , i.e.F-2 toxin, is an epiphyte toxin which has estrogen action produced by Gibberlla zeae, F. graminearum, Fudarium tricinctum and so on. It mainly empoison feed and cropper, such as wheat, oat, corn and so forth. It can arouse uterine portliness , metrohypertro -phia , non-oestrus and abortion of female livestock, female symptom of boar which have an enormously impact on the breed aquatics industry. Therefore, it is very necessary to probe into the influence of procreation caused by mutation of gene, and how to reduce the effect of ZEN on breed and growth of animals.Firstly, we injected different dosages of ZEN into rats, and distilled the DNA of different rates after 24h. The extracts were qualitatively and quantitatively appraisaled by 0.8% gelatin electrophoresis and measuring instrument. Secondly, by using PCR premier and PCR pre-reaction reagent box, we carried through the PCR reaction of on exon 8 of p53 gene, identified the pure of outcome by 1% gelatin electrophoresis. Thirdly, by SSCP analysis of the PCR outcomes, different ddDNAs were isolationed by SDS electrophoresis to measure whether ZEN has effected on exon 8 of p53 gene. At last, the outcomes were pured, and then sequenced. The sequencing were comparatively analysised by DNAMAN software to confirm the point of mutation.The result shows that the content of the pure extract of DNA is about 170ug/ml, which is fit for PCR reaction. And the molecular weight is about 200bp. The outcome of PCR is exclusive and about 200bp Mol. wt. ,which is very close to full size, therefore ,is the Exon 8 of p53 Gene, there are discrepancies in DNA strips of different groups. It means that ZEN had an effect on framework of the Exon 8 Mutation of p53 Gene. And then, the mutation of gene was discovered by measuring the sequence of gene.