Detectation And Polymorphism Analysis Of The Sheep Growth Hormone Gene By PCR-SSCP In Four Sheep Populations

Growth hormon(eGH)is one of the important factors in regulating many physiology and metabolic processes in animal body. So we detected the GH gene mutation in these 4 population such as Small-tailed Han sheep,Genmenmarino sheep ,Mgoulion sheep and hybrid generations of Genmenmarino and Small-tailed Han sheep by PCR-SSCP and DNA sequence analysis technology. The results was as following:1.By 5 primer, their were five locus were detected , which were the 1^st intron ,the 2nd exon , the 2nd intron, the 3rd exon , the 4^th exon , the 4^th exon ,the 5^th exon, in which , the 4^th exon was the polymorphic Loci.2.The analysis DNA sequence showed that both the mutation of G→A in 1257bp and T→C in 1405 bp in the 4^th exon took place,which resulted in the GH allelic gene mutate from A to B.3.Both the gene frequency and genotype frequency were calculated for the polymorphic Loci. The results were as following,:the frequency of A,B,AA,BB,AB, were 0.4858,0.5142, 0.2286,0.2571,0.5143 respectively inMongolian population; which were 0.3282,0.6719, 0.2188,.05625,0.2188 in Small-tailed Han sheep, 0.6055,0.3950, 0.4737,0.2632,0.2636 in Germanmerino , 0.5750,0.4113,0.4583,0.2947,0.2333 in hybrid generation 1 of Mongolian and the Small-tailed Han sheep and Germanmerino respectively. Moreover, the allelic gene A, B frequency inSmall-tailed Han sheep,Germanmerino,hybrid generation 1 of the Small-tailed Han sheep and Germanmerino were fit for equilibrium of Hardy-weinberg.4.The gene and genotype frequency analysis also showed that Germanmerino and hybrid generation 1 of the Small-tailed Han sheep and Germanmerino which have high mutton yield had the dominant genotype AA ,whileSmall-tailed Han sheepwhich have low mutton yield had the dominant genotype BB,.So we concluded that GH-exon4 might relate to the mutton yield of sheep.5.The polymorphisms analysis showed that Polymorphism information content(PIC)was medium, which from higher to lower wasMongolian(0.3748),Small-tailed Han sheep(0.3436),Germanmerino (0.3629),hybrid generation 1 of the Small-tailed Han sheep and Germanmerino (0.3883).The hoterozygosity (Ho)from higher to lower wasSmall-tailed Han sheep(0.5004), Germanmerino (0.5591), Mongolian (0.5226), hybrid generation 1 of the Small-tailed Han sheep and Germanmerino (0.4998). The heterozygosity (He) from higher to lower was hybrid generation 1 of the Small-tailed Han sheep and Germanmerino (0.4996), Mongolian (0.4409), Germanmerino (0.4774),Small-tailed Han sheep(0.5002).