| Canine adenovirus is adenoviride and adenovirus of mammalia genus. Its virulence is the strongest in adenovirus of mammalian. It includes type-1 and type-2. Canine adenovirus type-1 can result in epidemic hepatitis to dog and cephalitis to fox. And ordinary aspiratory ailment; Canine adenovirus type-2 can arise in epidemic laryngotracheitis and enteritis to dog. Weak bacterin is depressed farthest but can bring on ailment of eyes and kidney. Because there are crossed immunoreaction between canine adenovirus type-1 and type-2 and canine adenovirus type-2 can overcome shortcoming of canine adenovirus type-1 and inspire histogenic immunity and humoral immunity, it can protect animal from infecting of canine adenovirus type-1 and type-2 and canine adenovirus type-2. So canine adenovirus type-2 vaccinate against canine adenovirus all over the world now. Recombination bacterin which is carried by virus can admirably express external gene. Its production can enhance markedly histogenic immunity and humoral immunity give birth to immunocompetence. Gene of canine adenovirus have many excellence. Such as : its autoecious animal is excessive, infective efficiency is high,safe and steady, and convenient. Therefore, it become study hardcore of recombination bacterin and genic therapy .Now, it have acquired succesory that recombination bacterins of human, swine, cattle, sheep,avian is carried by adenovirus. For example, recombination bacterins of hydrophobic virus glycoprotein gene which was constructed human adenovirus type-5 could immunize from hydrophobia and acquired satisfactory effect.. But it can not well propagate in body of animal. That limited its adhibition; Canine animal is sensitive to CAV and have immunity itself. So, when external gene is inserted into it, it become 2-value genotypic vaccine. It can defend against type-2 illness. Recombination bacterin which carrier is used canine adenovirus type-2 have attract scientist for a long time. However, correlative report do not appear because its efficiency is low and its debug strategy is not clear. The study researched basic investigation which it is vitrol recombination between GFP and CAV-2. 1. After the genome of canine adenovirus type-2 (CAV-2) strain YCA-18 were acquired, its production were cuted by enzyme. B-segment which include E3 were callbacked. B-segment were cloned into pBluescriptIIKS(+) ,named pBSE3-2, E3 region sequence were determined and analyzed. The result show that E3 region of CAV-2YCA-18 are absolute the same as E3 region of CAV-2TorontoA26/61,Manhattan and SY. 2. What CAV-2 E3 region was got rid of is used pIRES1neo and GFP which included polyA.
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