Studies On Effects Of Exogenous Substance And Culture Conditions On In Vitro Plant Regeneration From Paulownia Elongate Leaves

Paulownia is a kind of popular,fast-growing trees which is of good quality,but those problems, including withes broom,large crown and short main stem and so on,keep Paulownia from further development.with the boom of modern science and technology,gene engineering and cell engineering provide effective methods to solve these problems.Researches about in vitro plant regeneration is basic for application of plants modern technology, in vitro plant regeneration has two ways, which are organ genesis and somatic embryogenesis.Setting up the system of in vitro plant regeneration of Paulownia lay soud foundation for gene engineering, cell engineering and cultivation of new cultivar of Paulownia.from our experiment of the system o ptimization of plantlets regeneration from Paulownia leaves by exogenous substance and culture conditons ,we got such following conclusions:1. Comparing the two kinds of carbon souces ,we came to the result that for the plant regeneration from Paulownia elongate leaves, sucrose was better than glycerol, and its corresponding optimal concentration is 20g·L^-1,and its corresponding bud induction rate is 64.15%.2. Both PVP and Vc could accelerate the plant regeneration of Paulownia elongate leaves,but their combinative effect was not better than their respective effect,for Vc,its optimal concentration was 0.05mg·L^-1, its bud induction rate was 69.23%.while the PVP optimal concentration was 7 g·L^-1, its bud induction rate was 81.58%.3. DTT was also good for the plant regeneration of Paulownia elongate leaves,and its optimal concentration was 10mg·L^-1, the higher or lower concentration did more harm than good,its bud induction rate was 65.72%.4. Compared with AgNO₃, citric acid was more effective to regenerate plantlets from Paulownia elongate leaves,and the citric acid best concentration was 0.15g·L^-1 in all the tested concentrations,its corresponding bud induction rate is 74.07%.5. With the existence of 10mg·L^-1 DTT in the media, the effect of PVP and Vc is different from the above,the best combination is AgNO₃ 0.015 g·L^-1 å’Œ DTT 10.00mg·L^-1,its corresponding bud induction rate is 65.22%.