Research On Identification And Molecular Phylogenetic Characterization Of Cryptosporidium From Poultry In Henan Province

Cryptosporidia are important zoonotic pathogen,we surveyed the epidemiology and research on the biologic characteristics of cryptosporidia for understand well the cryptosporidiosis in our country. The infectoion rate of Cryptosporidium were investigated in ostriches,quails,wild gooses and some species of zoo birds in Henan Province. The cryptosporidia infection percentage of ostriches and wild gooses were 2.48%(10/404), 2.99%(2/67) respectively, According to their morphological characteristics ,both of them were identified as C.baileyi; Two Cryptosporidia species were found in quail, they were identified as C.baileyi and C.meleagridis respectively, the infection rate of C.baileyi was 4.66%(20/429) and that of C.meleagridis was 10.02%(43/429), the total infection percentage of quail were 14.69%(63/429). The zoo birds were negative for cryptosporidium.Several cross-infection tests were performed by pathologic histology technique and scanning electron microscope technique. Oocysts of C.baileyi from quail were orally inoculated into quail and oocysts of C.meleagridis from quail were orally inoculated into quail, chickens, mice, ostriches, and ducks separately. Oocyst of C.baileyi from ostriches were orally inoculated into chickens, ostriches and mice respectively too. Main clinical signs of cough, asthma, sneezing, cachexia, dyspnea could be observed in the chickens and ostriches that infected with oocysts of C.baileyi, but there was no symptom as that of described as above in mice. There were only diarrhea in quail, chickens, mice, ducks which infected with C.meleagridis, but no clinical signs in ostriches.The experiments were carried out on pathology, ultrastructure of some organs in mice that infected with C.meleagridis form quail and in young ostriches that infected with C.baileyi from ostriches. We found that C.meleagridis derived from quail mainly parasitized in duodenum of mice and ileum of chicken which challenged with it, the intestinal villis of these experimental animal became atrophied and desquamated, C.baileyi from ostriches parasitized in cloaca and bursa of Fabricious of ostriches, mainly caused mucous membrane tumefaction.