Study On The Effect Of Inorganic Boron And Manganese Anti Black Bone Broiler Copper Poisoning

An experiment was conducted with 7×1 factorial design to study the effect of dietary copper on growth performance and clinical pathology indexes of black bone broilers. 280 day-old healthy broilers with an equal weight were assigned to 7 groups with 4 replicates at random, each having 10 chicks. The broilers were reared in cages. The house was controlled in constant temperature and maintained on a 24-h, constant-light exposure. The broilers were allowed accessing to the food and water at liberty. In trial, the broilers were fed with a basal corn-soybean meal diet supplemented with 50.9,200,400, 600, 800, 1000 mg/kg and 1200 mg/kg copper. Finally, the dosage of black bone broiler copper poisoning was received according to the result of this experiment.Then, 440 day-old healthy broilers were contacted with 2×2 factorial design to study the effect of inorganic boron and manganese anti black bone broiler copper poisoning. These chicks were assigned randomly to 11 groups with 4 replicates, each having 10 chicks which were fed with a basal corn-soybean diet supplemented with copper of poisoning dosages,60mg/kg B, 120mg/kg B, 200mg/kg Mn and 400mg/kg Mn. The clinical symptom, growth performance, pathological changes, clinical pathology indexes, liver function indexes and copper content in serum and liver of chickens were observed in order to estimate the effect of B and Mn anti black bone broiler copper poisoning.The experiment results indicated that: 1. The dosage of copper poisoning in black bone broiler was 600 mg/kg in meal diet. 2. The lead death dosage of copper in black bone broiler was 1200 mg/kg in meal diet. 3. As long as the content of copper in diet of black bone broiler was over 400 mg/kg, cumulating poisoning would occur. However 200 mg/kg copper in diet could promote black bone broilers growth. 4. Boron added in the corn-soybean diet of black bone broiler could alleviate copper poisoning symptom and postpone copper poisoning occurring. In addition, boron could protect liver cells and erythrocyte from damaging of copper. Foremost, Boron could reduce copper content in body. 5. The addition of manganese in the corn-soybean diet could aggravate copperpoisoning of black bone broiler, potentiate the poisoning role of copper against body in every respect and promote copper cumulating in black bone broiler, which showed that there was synergistic effect between manganese and copper.