Establishment Of Feline Panleukopenia Virus Diagnostic Method Of ELISA And HA/HI And Study On Its Immunity & Effect Of FPV Vaccine On The Panthera Pardus L.

The study was aided by the Ailuropoda Fund and designed on account of the Chengdu zoo' s topic that the Detection of the feline distemper' s serum antibody and the foundation of its immune programme about the grow wild feline animal such as Panthera pardus L, et al. The study regarded the attenuated feline panleukopenia virus as the diagnostic antigen which was isolated from the tri-combinant vaccine of Feline rhinotracheitis virus, calicivirus and panleukopenia virus . According to the tesults of Virus Physical and Chemical Character Detection, Agar Diffusion Reaction and Virion Shape Electron Microscope Observation, it was affirmed that the virion which was isolated from the tri-combinant vaccine was feline panleukopenia virus . Positive diagonistic antibody was made from the experimental animal which was immunized by single inactive FPV vaccine. The adsorption of the enzyme-labelled plate had been elevated about 80% by improveing the technique of ELISA. Furthermore,the sensitivity of ELISA had been elevated a lot when it was used to detecte FPV on clinic. Based on the related datum, we improved the techinique of HA/HI and established a suit of method which fitted the need for using on clinic. The results showed that the coincidence rate reached above 93% between ELISA and HA/HI. If ELISA and HA/HI were combined together when diagnosed FPV about the grow wild feline animal, it would not only overcome the shortcoming that the diagnosis of FPV was mainly depended on the epidemiology, the clinical symptom and the pathological change, but also elevate the sensitivity , the specificity and the facility of the FPV's clinical diagnosis.In order to detect the immunogenicity of the isolated strain, we injected it to the experimental animal.The results showed that it possessed favourable immunogenicity. It could induce forcefulness humoral immunity and cell immunity. The results that the Panthera pardus L. were immunized by the variety classes FPV vaccine showed that the P/N of ELISA was above 2 at the 36th week after the attenuated FPV vaccine was immunized and at the 23th week after the inactivated FPV vaccine was immunized. The datum would provide referenceable theory evidence when the people institute the immune programme for the grow wild feline animal.