The Study On Flower Developing,Tissue Culture And The Analysis Of Rapd In Anthurium Andraeanum Lind

Anthurium andraeanum is a very popularity top grad pot plant cultivars and cut flowers,with the development of ikebana artistic, Anthurium suddenly become the "dazzling star"in the rikebana realm.The same as "the world four cut flower"in the world flowers market,it is the secondly tropic flowers.Therefore there is very impotance significance to meeting the national flower market and export by studing and exploitation Anthurium andraeanum. The article studies on the process of flower development of Athurium andaeaum by using the general paraffin section technology and the electron microscope technology,to determining the cause of affecting fail seed,constructs regeneration system and analysis of RAPD of eight Anthurium andraeaum spcies were carried out.The primary results could be summarized as follows: The result of flower developing showed that the flower bud morphological differentiation way is form a leaf bud with a flower bud; The process of microspore occurring and male gametocyte development is normal,the development of anther wall is elementeny type.Thpetum is the gland cell type. The pistil has two carpels, the ovules belong to straight ovules with bitegmic;77% nucellus stoped development before from the archesporial cell and disappear and 23% nucellus atrophy .so the flower cannot grow seeds. The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L)。Eight lines belonging to species of Athurium andaeaum were analyed by RAPD markers to determine their relationship. Of the 30 primers screened, 12 primer no amplify bands,8 primer no polymorphic,10 primer showed consistent banding patterns and amplification and produced 81 bands with 46 polymorphic.The result of UPMGA clustered analysis as following: The study showed that the eight Anthurium cultivars examined were genetically closely related.The inheritance distance are smaller and the similarity conffifient are bigger.The result of clustering is consistent with Anthurium morphologic character .The study shows that RAPD can be a useful tool to distinguish Anthurium as well as identify their genetic relationships.