Construction Of Recombinant Fowlpox Virus Transfer Vector And DNA Vaccine Of GPV And GPMV

Goose parvovirus and goose paramyxovirus are the pathogens of violent fatal infectious diseases of goose existing nearly around the world. But the goose parvovirus is not reported in Africa and America yet. In recent years, with the extending of goose industry scale, two diseases became more and more harmful. When the diseases outbroke, frocks of gooses died rapidly without being treated in time. Germs and fungi disease often happened consequently. The diseases are contagious among goose of ages and breeds. High death rate of infected gooses, especially goslings, is the feature of these two diseases. There are not effective drugs which can control the diseases, so the research of vaccines are most important presently. The vaccines preventing GPV and GPMV in use now are traditional vaccines. With the development of genetic engineering technology towards the research of vaccine, genetic engeneering vaccine are becoming more and more popular hotspot, which is supposed to be more effective and safer as vaccines. In the research of genetic engeneering vaccine, foreign genes are cloned on the expressive vector or recombined into virus. After the vaccine is injected into the host's body, the foreign genes can express antigen protein which induces the immunity reaction. The two kinds of vaccines have the merits of their own: Studies on the recombinant fowlpox vaccine have longer history and are matruer in contrast with DNA vaccine. But DNA vaccine has the merits of safety,simple production technics,low production cost etc.besides the merits of traditional vaccines. With the thorough study on the it, DNA vaccine is becoming more and more perfect. It has become the symbol of the third era of vaccine. The choice of the experiment based on the background of the biology characteristics of GPV and GPMV and the trend of the research on vaccine currently. The experiment consists of two parts: (1) Gene VP3 of GPV is amplified and cloned into the expression vector pcDNA3.1(+) to construct the recombinant vector pcDNA3.1-VP3. Then the fragment including promoter Pcmv,gene VP3 and BGHpA on pcDNA3.1-VP3 is amplified and cloned into the recombinant vectors pcDNA3.1-F and pcDNA3.1-HN to construct the double-gene DNA vaccines vectors pcDNA3.1-VP3-F and pcDNA3.1-VP3-HN. (2)The fragment including the promoter LP2EP2 and gene VP3 on the recombinant vector pSY538-VP3 is cloned into the recombinant vectors pSY681-F and pSY681-HN to construct the double-gene recombinant fowlpox virus transfer vectors pSY681-VP3-F and pSY681-VP3-HN. The construction of recombinant vector is the prophase work in the research of genetic engineering vaccine, and also the key ensurance of efficiency. The anaphase immunity work can be proceeded only on the basis of possibiliby of effective expression on theory of foreign gene. Wether the vaccine could induce satisfactory immunity reaction depends on many conditions. Four constructed recombinant vectors esbablished the base for genetic engineering vaccines which prevent diseases aroused by goose parvovirus and goose paramyxovirus.