Inheritance Of The Resistance To Stripe Rust In Wheat Variety Chuanyu16 And The Molecular Discrimination Of High Molecular Weight Glutenin Subunits

Wheat stripe rust is one of world-wide key diseases. This disease appears seriously in southwest and north areas of china, which affects high and stable yields of wheat. The best way to control wheat stripe rust is breeding and using resistant cultivars.Wheat variety Chuanyu16 possesses many good traits such as earliness, high-yield, white-grain and big-grain. According to the results of resistant investigated conducted by Chinese Academy of Agricultural Sciences and Sichuan Academy of Agricultural Sciences. Chuanyu16 is also highly resistant to the current stripe rust races. It is considered a desired resistant variety. In order to make use of this variety , we carried out the study on inheritance of resistant gene in Chuanyul6. Based on the parents, F₁, F₂ and BC₁ progeny resistance reaction to stripe rust race CYR31 and CYR32 and the resistance segregation ratio respectively, it is concluded that the Chuanyu16 resistant to race CYR31 was controlled by one dominant gene, and the resistance to race CYR32 was controlled by the complementary action of one dominant gene and two recessive genes. Stripe rust resistance of Chuanyu16 is mainly controlled by dominant nucleic genes. Especially it shows highly resistant to current stripe rust races at plant adult stage, which is much useful in wheat disease resistance breeding. Since the reaction phenotype of Chuanyu16 was different to that of the parents, it is not confirmed where the resistance gene is derived from.Wheat high molecular weight glutenin subunit (HMW-GS) 2, 2.2and 5 is controlled by genes located in chromsome1D. They make great contribution to wheat quality. In this paper, we carried out study on the molecular discrimination of HMW-GS 2, 2.2and 5. According to the published gene sequences encodingHMW-GS 2.2 and HMW-GS 2, we made alignment of their nucleotide sequences. 1 pairs of PCR primers was designed. A target DNA fragment was amplified to distinguished 2.2 from2. Adding the particular PCR primer for 5 and optimizing the PCR condition, a multiplexed PCR system was established to distinguish 3 major HMW-GS in a single PCR reaction and agarose electrophoresis. The two pairs of PCR primers can simultaneously identify HMW-GS 2.2, 2 and 5 in a single PCR reaction. The multiplexed PCR method was proved by the assay result of other wheat varieties. PCR result was consistent with the genotypes of the varieties identified by SDS-PAGE .The molecular markers for HMW-GS 2.2 is developed and the multiplexed PCR is established. It can be employed to distinguish three HMW-GS in a single PCR reaction. Molecular markers have many advantages over other kinds of marker. Using molecular markers, it is quickly and properly to identify the HMW-GS composition of the hybridization progeny. This method shows excellent applied property in marker-assisted selection for wheat quality breeding .