Effects Of Active Immunization Against The Graafian Follicle Inhibin On The Reproductive In The Meat Sheep

Gather the fresh swine ovary in vitro, collect the follicle fluid under 4 °C conditions. Dispose theswine follicle fluid under 4 °C conditions with the activated carbon for 24 hours, in order to fully adsorband eliminate the steroid hormone in follicle fluid, centrifugation eliminate activated carbon, join in -20℃ anhydrous alcohol, enable the density of alcohol to achieve more than 86% (V/V) , mix symmetricalin order to deposit the protein ingredient in the follicle fluid fully, gather the deposition at the bottom ofthe tube, dissolve obtained deposition in 20mL0.01mol/L,pH7.0 PBS, then carry on the SephadexG-100gelatin to filter the chromatographic analysis, in chromatographic analysis process, record the twoproteins peaks by the nucleic acid protein instrumentation, which marked as A peak and B peak, collectthe products of the two peaks separately, with the SDS- polypropylene acid radical ammonia gelatinelectricity swims, and with the aid of standard protein and swine follicle fluid inhibin standard analysisthe products of the two peaks , the experimental result indicates the inhibin is mainly in the A peak.Through the hCG ovary increases restraint method mensurate the inhibin activeness of the products offilters the chromatographic analysis, The hCG ovary increases restraint method is one kind of simple,facile, quickly inhibin biological activity determination method. The experimental result indicated Apeak has the stronger inhibin biological activity, seriously has the obvious suppression effect on theovary increases to hCG;B peak and the swine follicle fluid has no serious suppressed the effect on theovary increases to hCG. Join the freund's adjuvant into the A peak freezing and drying matter to makethe immunity antigen and carry on three times of immunities to the meat sheep, thereinto the first timeimmunity is used completely the freund's adjuvant, and the two times strengthen immunities are usedincompletely the freund's adjuvant. This experiment has designed one control team and three differentmonitoring teams respectively are 1 ml monitoring team, 1.5 ml monitoring team and 2 ml monitoringteam, the result discovered, in the control team five sheep's blood serums has not examined the inhibinimmune body. In the three different monitoring teams ,10 days after immunity could examine theinhibin immune body besides the individual experimental sheep, but the immune body titer is low, andbefore the secondly immunity the immune body level have reduced. After the strengthen immunities theimmune body level in blood has elevated gradually, but the 1 ml monitoring team and the 1.5 mlmonitoring team produce the immune body level were still low, only in the 2ml monitoring teamexperiment the sheep after two times strengthen immunities produce to become the higher effectiveprice to be even and stable inhibin immune body. Before the first time immunity, in various monitoringteams and the experimental control team the blood serum FSH density difference is not remarkable, 2mlmonitoring team's FSH density on remarkably is higher than the control team after the first immunity,and the 1ml monitoring team and 1.5ml in the monitoring team blood serum FSH density although hasslightly elevates, but to the control team sheep's the FSH density in blood serum, the difference is notremarkable. After two times strengthen immunities, although in tests 1 and tests 2 groups of sheep bloodserums the FSH content are higher than control team slightly, but the difference is not remarkable. After the third immunity, observe ovary change through the surgery method , obviously the ovary assumes light pink, above which has bright red corpus luteum and the transparent follicle, on the individual sheep ovary has corpus albicans to exist, the 2ml monitoring team's corpus luteum number and the bigger than 3mm follicle number are remarkably to be higher than the control team. But the 1 ml and the 1.5ml monitoring teams have slightly elevates but after the statistical software analysis ,the difference with the control team is not remarkable. The meat sheep immunised with antigen I 2ml which obtained in this experimental system, and then immunized with antigen II 20 and 40 days after the first immunity as the two times strengthens immunity, may effectively enhance the corpus luteum number and growth follicle number, and also could primely enhance the reproduction performance.