Study Of Improvement And Genetic Diversity Of Soybean Male Sterile (ms₁) Recurrent Selection Population

A mostly limiting factor of soybean breeding is genotypic straitness. This problem can be overcame through recurrent population, in theory. In order to evaluate the effect of recurrent selection of the population, the genetic diversity of 3 soybean ms₁ sterile recurrent selection population was studied in phenotype and molecular level. The main results were as follows:1. For the high oil (gy) population, the mean of oil content was 20.23% and the highest achieved 22.59%. While the high protein (db) population, the mean of protein content was 43.70% and the protein content of 22.38% plants achieved 45% at least. Those two quality characters were higher than that of LD population(the base population), so the improvement was successful.2. The per plant yield of two improved recurrent selection population was much higher. For the high oil population, especially per plant weight was 14.0g and 100-seed weight was 18.4g. For the high protein population, per plant weight was 12.9g and 100-seed weight was 19.2g.3. In morphological and molecular level, it seemd that the genetic diversity of 3 ms₁ sterile recurrent selection population soybean were more than landrace and bred varieties of soybean in Hebei in some extent. Both oil population and high protein population had higher genetic diversity than the base population (LD population) either in morphological or SSR molecular level.Based on morphological data, for oil population, the variation coefficient was 36.38% and Shannon-Weaver information index was 1.783;for protein population, the variation coefficient was 36.03% and Shannon-Weaver information index was 1.748.Detected by 39 SSR primers, the alleles number of oil population was 365, and Shannon-Weaver information index was 1.652. For oil population detected by 39 SSR primers, the alleles number was 370, and Shannon-Weaver information index was 1.705.4. Genetic diversity based on SSR data was not consistent with that determined by agronomic traits, and more genetic information would be obtained from SSR data associated with agronomic traits. And either in morphological or SSR molecular levelclustered result, the genetic background of recurrent selection population was different from the Iandrace varieties of soybean in Hebei.