The Study Of Relative Adhesive Proteins Of Lactobacillus In The Paralichthys Olivaceus Intestine

Adhesion is a progress of interaction between the bacterial surface componentsand host cell surface, and the first step of colonization of bacteria. It is bases on therelative adhesive components of the bacteria and intestine, research on thecomponents is vital to the adhesion mechanisms of the probiotics to the intestine,additionally, and the further study is important to the microzoology provement inthe intestine and the zoological prevention to the pathogen. The colonization of theadhesion to the intestine of the Lactobacillus can inhibit the pathogenic bacterialinfection, therefore play the role of bio-resistence-bacteria.Lactobacillus L15 was isolated from the intestine of Paralichthys olivaceus.Weinvestigated the adhesion of the new strain Lactobacillus L15 and other kinds ofLAB by the mucus model in vitro. The results showed that different strains withdifferent adhesion. The strains tested all can adhesion to the mucus of farmedflounder(Paralichthys olivaceus),Common carp, mouse and collage. L15 showedgreatest adhesion to the mucus of farmed flounder, and the L. acidophilus to themucus of mouse, Extracting the surface protein and the mixing the extraction ofsurface protein, L15 and L. acidophilus all showed obvious reduction to the mucus.We found that the adhesive proteins were involved in the extraction of the surfaceproteins. LAB and the extraction of surface proteins can inhibit the pathogenicbacteria obviously. The results indicated that the adhesion is bound to strainspecificity, and the origins of the strains can affect the adhesion too. In addition therelative components of adhesion exist on the bacterial surface, this result isaccording with the relative reports.After Western blotting,we got different proteins from foregut mucus ofParlichthys olivaceus and membrane .There are two protein bands which weight30.3kDa and 29.7kDa both in intestinal mucus and membrane, but there isredundant protein which weight 12kDa in membrane. There are two protein bandswhich weight 30.3kDa and 29.7kDa in hindgut, intestinal mucus and membrane, butthere is no protein in caecum. We can conclude that the LAB have the strainspecificity and the host specificity. Different hosts showed different adhesivereceptors, even the different parts of the same apparatus showed different receptors.Although the results is new, it is consistent to complexity of adhesion between thebacteria and the intestinal mucus.The location of tissue and the acid glycoprotein were showed clearly by theparaffin section stained with HE ,PAS and AB-PAS of the farmed flounder. L15labeled with FITC showed a kind of lactic bio-membrane, we can concluded that thereceptors is located on the mucus surface of intestine of the farmed flounder.