The Study Of Relative Adhesive Proteins Of Lactobacillus In The Carp Intestine

Adhesion, the first step of colonization of bacteria, is a progress of interaction between the bacterial surface components and host cell surface. It bases on the relative adhesive components of the bacteria and intestine. It is vital to study the components for the adhesive mechanisms, additionally, the further study is important to the microzoology provement in the intestine and the zoological prevention to the pathogen. The colonization of Lactobacillus to the intestine can inhibit the pathogenic bacterial infection, and play the role of bio-resistence.We identified and analyzied the receptors in the commom carp for the Lactobacillus acidophilus ATCC4356 and Lactobacillus L15, the latter was isolated from the intestine of healthy Paralichthys olivaceus. The location and distribution of the protein in intestinal epithelia were tested too.After western blotting by surface proteins of L15 and L. acidophilus, we got the same protein from foregut and midgut in carp intestine. There was a protein band the moleculer weight was 26.2kDa in intestinal mucus. In hindgut, the components are very different from foregut, and there was no special protein in it. We got antiserum from the mice, which immunitied by above protein. The antiserum by 200 multiple dilutedness could combine specially with the receptor of carp mucus. And it was concluded by the result of western blotting that there was the same protein which moleculer weight was 26.2kDa in foregut and midgut of carp. Though it was different surface proteins between L15 and L. acidophilus, they had same adhesive receptor in carp intestine.By the experiment of adhesion, we studied the function of the receptor in adhesion of test strains to carp mucus. The results showed that the three test strains could adhere to the carp mucus. And the antiserum could inhibit adhesion of the test strains. We concluded that the protein was adhesive receptor in intestinal mucus of common carp. Probiotics and pathogenic bacteria could adhere to carp intestinal mucus, and they were likely to compete the same adhesive receptor and it was the mechanism that lactobacillus inhibits the pathogenic bacterial infection.The location of tissue and the acid glycoprotein were showed clearly by the paraffin section stained with H.E. and AB-PAS of common carp. We observed carp intestine from the tissue and cell levels, and mastered its construction. By the immunity histochemistry of paraffin section from carp intestine, we concluded that the adhesive receptor of lactobacillus was secreted by intestinal epithelium colloblast, and located in foregut and midgut.