| Cotton Verticillium wilt, a disease of cotton caused by Verticillium dahliae, is a kind of worldwide spread disease that is difficult to be controlled in cotton production. Development of cotton resistant cultivars can provide an effective and economical method for controlling Verticillium wilt. Therefore, researches foused on deeply understanding of expression rules about resistance gene and the molecular mechanism about interactions between Verticillium dahliae and the host would provide theoretical foundation for developing resistant cultivars with gene engineering.The objectives of the research were attempted to obtain resistance-related candidate genes from three cotton cultivars induced by severe-virulence strains of Verticillium dahliae, which would provide new gene resources for cotton resistance breeding. The research selected 31 from 256 primer combinizations, which found out 82 differential display fragments. There were 55 bands reamplified, selecting 15 bands to clone and sequence. The clones were named Vdrg (Response Gene induced by Verticillium dahliae).The cDNA in the study showed no detectable homology to the known cotton library, suggesting that were probably represent fragments of novel genes induced by Verticillium dahliae. They were compared to genome libraries, and the results indicated that Vdrg10 was a 337bp gene fragment that was 99%(318/319) identical to putative transport protein from Shigella boydii;Vdrg28 was a 323bp gene fragment that was 91%(243/266) identical to 50S ribosomal protein L3;VdrgAl was 99% identical to protein tyrosine kinase from E.coli, and it was a 305bp gene fragment;Vdrg33 was a 291bp, which was 84%(174/207) identical to putative clathrin binding protein from Arabidopsis thaliana. The relationship of the others and the known resistant genes is uncertain, and it is expected to research in the future.
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