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Nematicidal Activities Of Several Plant Extracts Against Meloidogyne Incognita And Separation Of Active Ingredients

Posted on:2007-08-02Degree:MasterType:Thesis
Country:ChinaCandidate:Q DingFull Text:PDF
GTID:2133360185450479Subject:Pesticides
Abstract/Summary:PDF Full Text Request
Recently, more attention has been paid on products of natural plants which play an important role for impoldering substitutes of organic synthetic pesticides which are serious threat to the environment such as resistance, residue and resurgence, especialy high toxicity to vertebrates. In order to develop new ways and new natural products for controlling Meloidogyne incognita, nematicidal acitivities of 8 species plants to Meloidogyne incognita were studied with bioassay both in vitro and in vivo in present paper. Nematicidal components of Chrysanthemum coronarium were also initially separated. Meanwhile, nematicidal acitivities of 13 phytochemicals were also investigated, and the results would provide some theoretical basis for the research and development of friendly nematicides. The results could be summarized as follows:1. In vitro bioassay results showed that, methanol extract(MeOH-Ext.)of Chrysanthemum coronarium (20mgDPP/mL) had the best nematicidal activity to J2 of Meloidogyne incognita in all the extracts with ajusted mortality: 91.78%(24h), 87.70%(48h), 85.34%(72h). But the bioactivity of MeOH-Ext.of Chrysanthemum coronarium was debasing with time prolongating, it may be illuminated that the pertinence between bioactivity and time was negative. The results also indicated that the nematicdal components mainly existed in the strong polarity solvents extracts.2. In vivo bioassay results showed that, the number of root-knots could be effectively reduced dipping tomato seedlings with MeOH-Exts of Chrysanthemum coronarium, Cirsium setosum, Datura stramonium, Ricinus communis, Chenopodium album, ethyl acetate extracts(EtOAc-Exts.) of Lantana camara, Chenopodium album and petroleum ether extract (PtOEt-Ext.)of Chenopodium album for 30 minutes before transplanting, and the control effects of MeOH-Exts of Chrysanthemum coronarium and Ricinus communis were 90.83%, 73.17% respectively. J2 amounts could be availably restrained by MeOH-Ext. of Chrysanthemum coronarium, PtOEt-Exts of...
Keywords/Search Tags:Chrysanthemum coronarium, Meloidogyne incognita, bioactivity, phytochemicals
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