| The Psathyrostachya perennis Keng.cv.shandan were used for the study of tissue culture regeneration system and polyploid induction. By study and filtration the best hormone assorted proportion of calli induction culture ,multiplication culture and differentiation culture of mature embryo,which established regeneration system from callus to regeneration plantlets; studies on chromosome doubling by colchicines liquid treating germinating seeds.embroy.diploid seedlings.calli and regeneration plantlets, discussing the suitable treatment concention and time of different induction material. The polyploid plants of Psathyrostachya perennis Keng.cv.shandan were obtained. The results were as following:(1) The optimal hormone assorted proportion for inducing mature embryo callus was MS+2.4-D 2.0 mg/L. Culture and the germination rate was 91.7%.(2) The optimal hormone assorted proportion for multiplication culture was MS+2.4-D 1.5 mg/L+KT 0.5 mg/L.The daily average multiplication rate was 1.43%.(3) The optimal hormone assorted proportion for differentiation culture was MSå2.4-D 2.0 mg/ LåNAA 1.0 mg/ L.The green bud rate was 78.5% and seedling rate was 49.5%, while the albinism rate was only 2.5%.(4) The doubling effect was different obviously by chromosome treating with the different materials. The doubling effect was best and the the induction frequency vas highest 62.5% by treating diploid seedling , the induction frequency vas 50.0% by treating embroy and callus , while the induction frequency vas lowest 14.3% by treating germinating seeds .(5)All plants with doubled choromosome number were mixoploids including diploids (2n=2x=14) and tetraploids (2n=4x=28), there are choromosome of 2n=3x=21 in individual doubled plants.(6) The leaves of the doubling plants became lomger and wider obviously, and they were 123.80% and 102.97% of the control , the plants height was 126.47% of the control respectively .
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