Study On The Molecular Mechanism Of Cytoplasmic Male Sterility In Near Isogenic Lines Of Restoring Gene To Wheat V-CMS

Cytoplasmic male sterility (CMS) is a maternally inherited trait in which higher plants fail to produce functional pollen, but maintain female fertility, it has been observed in over 150 plant species. CMS is coordinated by nuclear and cytoplasmic interactions. The mechanism of CMS is closely associated with cytoplasmic inheritance, and it is an important domain of genetics. The CMS trait is useful in the production of hybrid seed and heterosis utilization, because it greatly reduces the cost of hand emasculation. As one of the major crops in China and all over the world, wheat plays very important role in the development of Chinese national economy and social stability. Utilization of heterosis is one of important and useful ways to promote crop output and quality. Male sterility with Aegilops Ventricosa cytoplasm has been proven to be promising in hybrid wheat breeding, which has broad spectrum of restoring gene resources and has no distinct harmful cytoplasmic effect. In this paper, with near isogenic lines of restoring gene as materials, serial molecular techniques are adopted to study the molecular basis of V-CMS wheat, the major results are shown as follows:1. With near isogenic lines of restoring gene to wheat V-CMS as materials, the mtRNAs of male sterile line, maintainer line and F1 hybrid were compared by cDNA-RAPD method. Two hundred and eighty-four primers were screened, 104 of them had polymorphisms between male sterile line and maintainer line, and 22 primers had polymorphisms between male sterility line and F1 hybrid. A fragment of S238999 specifically expressed in cytoplasm male sterility line, was proved to be differential among cytoplasm male sterility line, maintainer line and F1 hybrid by Northern hybridization, which inferred that the fragment...