| The experiment was conducted from 2004 to 2006 in the orchard and hormone laboratory, College of Horticulture, Shandong Agricultural University. Annual apple seedlings and 3-years-old trees of Malus hupehensis (Pamp) Rehd. were used as materials. Effects of some plant growth regulators on the regulation of root formation and development were studid, and the physiological mechanisms of IBA, ETH, PP333 and GA in regulating root formation and development were discussed. The main results were as follows:1.α-NAA, PP333,JA,SA all could improve the ability of root formation in apple tree, the best contents of each are10,5,12,8 mg·L-1,and each treatment of this content enhanced the number of lateral root primordium by 302.84%, 105.30%, 155.45%,225.83%.2. IBA could increase the lateral root primordium number of Malus hupehensis (Pamp) Rehd. Effects of roots treatment on inducing lateral root primordium formation was better than leaves treatment,the number of lateral root primordium was 6.33 ,2.11 times as many as those of CK and leaves after 96 h of treatment.3. IBA also could change the POD activity, the POD activity of Malus hupehensis (Pamp) Rehd.seedling roots increased by 90.30% to the biggest in 1 h, when the POD activity in leaves first was decreased,and then increased.4.The protein kinase,phosphatase and H+-ATPase activity of the extensive roots were all bigger than those of absorbing roots.The IOD and POD activity order was brown roots biggest, extensive roots was secondly,and the absorbing roots was lowest. The lateral root primordium formation was relative to protein phosphorylation and H+-ATPase.5. AgNO3, the inhibitor of ETH receptor restrained the effects of IBA on advancing the roots development, promoting the ATPases and protein kinase activity. But using AgNO3 alone could raise the length per gram, number of tips per mm, number of tips per gram and square per gram of roots. 6. PP333 could promote the root overstriking-growth and root tip swelling,...
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