Studies On Plasma Membrane ATPases' Regulation In Malus Hupehensis (Pamp)Rehd. Roots And The Relationship Between ATPases And Root Nutrition Uptakes

The changes of plasma membrane H+-ATPase and Ca2+-ATPase activities, protein overall kinases activities and root nutrition uptakes were studied under treatments of 4 kinks of nutrition elements, 4 kinds of plant growth regulators and root pruning as well as base fertilizer, using annual seedlings and 2-3 years old trees of Malus hupehensis(Pamp)Rehd. as materials, in Hoagland nutrient solution, sand and nursery basin. Moreover, via inhibitors experiments and regression analysis, the relationship between PM ATPases and root nutrition uptakes was also researched. Results were as follows:1. NaH2PO4,KCl,Ca(NO3)2 and MgCl2 could significantly activate PM H+-ATPase and Ca2+-ATPase. NaH2PO4,KCl,Ca(NO3)2 could also enhance the PNPP hydrolysis activity of PM H+-ATPase and reduce the Km value between H+-ATPase and PNPP.2. NaH2PO4,KCl and MgCl2 could increase the activities of PM protein overall kinases, which had evident relationship with ATPases activities.3.Under treatment of NaH2PO4, the root uptake rates of Fe,Ca,Mn,Zn,Cu were remarkably correlative with PM ATPases activities; the changes of root uptake rates were similar with the trend of protein overall kinases activities, but there was no relationship between them.4. White roots'reactions to plant growth regulators were different with that of brown roots and the former was more sensitive. Indoleacetic acid (IAA,10-80μg/g) andα-naphthyl acetic acid (NAA,10-40μg/g) could activate PM H+-ATPase and Ca2+-ATPase of white and brown roots in 2-3 years old trees, and the inducing effect was more evident in the former; while high concentration of IAA(100μg/g)and NAA(50μg/g) inhibited brown roots...