Stage And Gender Differential Proteome Of Schistosoma Japonicum

Schitosomiasis, caused by schistosome, is the wide spread zoonosis that seriously theatened the human and animal health. Schistosome exhibites complicated characteristic growth, development and repliction. During its life cycle, schistosome present dramatic changes in its biology and morphology. It is believed that these biological and morphological changes are accomanied by differential proteome at the various stages, which result in special metabolism and development. Based on the above features, studies on the different proteome amang cercariae, schistomula, egg, male and female Worm in Schistosoma japonicum were carried out.Soluble proteins and hydrophobic proteins that were extracted from cercariae, schistosomulum(8 days and 19 days), adult male and female worm (42days), eggs of Schistosoma japonicum were separated by two dimensional electrophoresis. There were 928±61, 1465±41, 1230±30, 904±34, 1080±26, 1376±52 spots for soluble proteins, 845±53, 986±22, 1145±35, 1066±39, 1123±45, 1437± 44 spots for hydrophobic proteins from cercariae, schistosomulum(8 days and 19 days), female and male worms of adult stage(42days), egg, respectively. A high degree of quantitative and qualitative similarity in spot pattern was revealed across the life-cycle, greatest between adjacent stages. MALDI-TOF/TOF MS and LTQ MS were employed to analyze 95 differential or ubiquitous spots for soluble proteins, 84 spots were idendified successfully for peptide mass fingerprinting and sequencing, including 29,16,18, 12, 9 spots from schistosomulum(8 days and 19 days), female and male worms of adult stage(42days), egg, respectively. These proteins comprised 75 different protein species. Lastly, 3 differential spots and 1 ubiquitous spots were idendified by RT-PCR. The results showed that a series of intracellular signal molecules, molecules involved in cellular metabolism, cell motility, protein folding, protein biosynthesis, protein modification and transcriptional regulation were expressed differentially.Our result are useful for better understanding schistosome special mechanism of growth, repliction and interaction with host on molecular level, and to open a new path for preventing and curing schistosomiasis.