Preliminary Studies On Differential Proteins Of Flower And Leaf Buds In Longan(Dimocarpus Longan Lour.)

The mechanism of bud differentiation in fruit trees is one of the basic theories for fruit tree cultivation. Studying on fruit tree bud differentiation is the important content of the fruit tree physiology, and is the focus topic of the fruit tree development biology. It has close correlation to production, and is closely related to overcome the big and small year outcome, to improve fruit character and make young trees bear fruit ahead of time. Logan flower buds are the basis of longan flowers and fruit formation.The present study investigated the proteomes of Longan flower and leaf buds. 2-DE, coupled with MS and protein database searching, was used to separate and identify the differential proteins of flower and leaf buds in longan. Ten differential proteins up-regulated were identified by MALDI-TOF-TOF/MS. These proteins were categorized into classes closely related to scavenging and anti-oxidation of free radical, amino acid metabolism, signal transduction and basic metabolism, substance and energy metabolism, environment press resistance and other physiological processes in the development of Longan flower and leaf buds. These ten proteins were peroxidase, ascorbate peroxidase, Protein group similar to late embryogenesis abundant proteins, 14-3-3 protein, 14-3-3 family protein, LHCII type I chlorophyll a/b binding protein, ATP synthase beta subunit, fructokinase, putative cytosolic cysteine synthase 7, retrotransposon protein, putative, Ty1-copia subclass. The identification of these proteins provides new insights that can lead to a better understanding of the molecular mechanism of bud differentiation in Longan.