The Culture And Differentiation Of Morel Mycelia

Morels are rare wilding fungus and famous for the outstanding value both in pharmacology medical uses and edibleness. In this paper, strains were obtained by tissue isolation and single spore isolation. By morphology and molecular biology, six strains were selected to study. We observed the cultural characteristics of hybridization and secondary culture of morel mycelia of M. angusticeps and M. crassipes. The result showed that the hyphae from the above two species of Morchella can not occur hyphal fusions. We researched the influences on the mycelial growth of Morchella sp. produced by Trichoderma sp. and Valsa sp. which from tissue isolation of Morchella sp. and found that the extractions from Trichoderma sp. and Valsa sp. can accelerate mycelial growth of Morchella sp.. The association between morel and plant was discussed; the results indicated M. angusticeps can form premycorrhizas with the tissue culture seedlings of Populus popularis , but this mycorrhizic types lacked of mantle and Hartig net; and sclerotia by co-cultivation of M. angusticeps and P. popularis enlarged distinctly. The technique of preparation and regeneration of protoplasts from Morchella sp. was built and the optimal conditions for preparation of protoplasts from M. crassipes were examined; the largest numbers of protoplasts were obtained with mixture snailase(4mg/ml) and lywallzyme(5mg/ml) after hypha cultured for 3 days, 30℃ , 50rpm/min and 3 hours enzymolysis in 0.6M NaCl osmotic stabilizers.