Studies On Preparation Of Fucoidan Oligosaccharide And Its Role In Eliciting The Cold-resistance

Chilling stress offen occur in agriculture, which cause a drastic declive in yield and quality of crops, and make large of loss, so it is urgent to study the cold resistant mechanism of crops and manufacture an available cold resistant drug for crops. Exogenous materials can improve cold-resistance capability of plants. In this paper .fucoidan oligosaccharide were prepared and its abilities of eliciting the cold-resistance capability of crops were studied.(l)Fucoidan were exacted by acid from browm alga (Laminarin Japomica), and were perifide by ethanlo and DEAE-cellulose ion-exchange chromatogragh. The fucoidan was partial hydrolyzed with HCl.The products were isolated and desposited by ethanol, fucoidan oligosaccharide with average degree of polymerization 8-20 were further separated through sephdexG-25 gel filtration.(2)The effect of fucoidan oligosaccharide on crops seeds germination were studied. The results show that 0. 01%-0.1% fucoidan oligosaccharide solution on the crops can increase germinating pecemtage, germinating index and vigor index of cabbage maize tomato seedlings. 0. 1% fucoidan oligosaccharide solution is the best.(3)0.01%-0.2% fucoidan oligosaccharide solution all can incress cold-resistance capability of plants, the effect of different standard of the same drug are different. 0.1% fucoidan oligosaccharide solution is the best.(4)The cold-resistance susceptivity of different crops of the same drug are different. As far as 0.1% fucoidan oligosaccharide, first one is cabbage, nest is maize, the last is tomato.(5)Fucoidan oligosaccharide to improve the ability of crops cold resistance mainly by enhancing the activity of POD and SOD, decreasing pxidation of cell membrane and its pruduct MDA, increasing the comtent of dissolubility protein and soluble sufar, reducing the decomposite speed of chlorophyll and the leakage of electrotytes decrease.