The Processing For High Yield Of Active Compounds In Suspension Culture Of Cistanche Derserticola And Developing Of Functional Drinks

The callus of Cistanche deserticola was induced from wild stem explant and the in vivo screening for interesting culture was used. Then a stabilized cell suspension culture was established. As the object of the callus of Cistanche deserticola with high yield of phenylethanoid glycosides (PeGs) and the targets to gain the higher biomass, the utilizations of sucrose, nitrogen and phylosphate scourse were observed on the basis of B5 medium, and the metabolized kinetics of regulation of biomass, protein, starch and secondary metabolized products were investigated. The chitosan, oligosaccharide and bentonites were used as elicitors to promote biosynthesization of PeGs in the suspension culture of Cistanche deserticola. The purification and identification of PeGs were carried out. On the base of these researches, the series functional drinks were exploited. The results are as follow.I. Using stem buds of C. deserticola as explant materials, the calli were induced. In view of browning caused by polyphenoloxidase and by the study of inhibition by citric acid, Na2SO3 and Vitamin C, the optimized medium for the callus subculture is B5+IAA 1.0mg L-1+ KT 4.0mg L-1 + Vitamin C 3.0mg L-1 +actived carbon 1.0 g L-1, which can remarkably inhibit browning of C. deserticola cell and improve the biomass. The relative increment was 0.02 g DW mL-1.II. Based on establishment of C. deserticola suspension system, the growth of C. deserticola cell, nutrition substrates and second metabolized products were studied. The results showed that the consumption of carbon and nitrogen scources and the biosynthesis of PeGs were slow during the suitable phase (0-5d), but the consumption of carbon and nitrogen scources and the biosynthesis of PeGs were accelerated rapidly during the active phase (6-20d), and the growth of cells and biosynthesis of PeGs slowed down during during the stationary phase (21-30d). During the stationary phase, the proteins, inorganic salts and secondary metabolized products were released.III. Then regulation of initial sucrose and nitrogen concentration and NO3-: NH4+ were put in practice. The experimental results showed that the cell growth reached its peak of 24.18 g L-1 DW and the content of PeGs was 20.65 % (DW) under an initial sucrose concentration of 30 g L-1, NO3-: NH4+= 32:0 and KT4 mg L-1+ IAA0.5 mg L-1.