Study On The Antibacterial Activity And Resource Utilization Of Magnolia Officinalis Rahd.et Wils.var. Biloba Rehd.et Wils. Growing In Fujian Province

Because of botanical fungicide from the plant is natural, safe and hard to cause drug-resistance, researching and developing it is becoming a hotspot in current society. We study the cortex Magnolia officinalis Rahd. et Wils. and different parts of Magnolia officinalis Rahd. et Wils. var. biloba Rehd. et Wils. growing in Fujian province and estimate the antibacterial activity of them by Kirby-Baueer Disc Diffusion assay. The crystals of antibacterial active components part I and part â…¡ were distilled respectively. The content of magnolol and honokiol in M. officinalis and different parts of M. officinalis var. biloba was measured. Results as follow:1. Experiments proved that active components which can inhibit Escherichia coli, Staphylococcus aureus , Bacillus subtilis and Salmonella strongly consist in cortex M. officinalis and different parts of M. officinalis var. biloba growing in Fujian province by antibacterial activity assay. Different solvent extracts all have the antibacterial activity, which indicate that there are different polarity components taking a role in antibacterial activity. In all the different solvent extracts, petroleum ether extract with fewer impurities is easy to be purified while ethanol extract with more impurities is better for crude extract.2. The extraction and isolation of active components from cortex M. officinalis and active-guided isolation assay indicate that antibacterial active components in cortex M. officinalis are crystals part I and part â…¡. They are identified as magnolol and honokiol by a series of experiments such as measuring melting point, TLC, scanning by UV-visible spectrophotometer and infrared spectrophotometer with the standard substance. The minimal inhibition concentrations (MIC) of partI against S. aureus, B. subtilis, Salmonella and E. coli are 20, 30,...